Journal: Scientific reports

Article Title: Ciliary neurotrophic factor is increased in the plasma of patients with obesity and its levels correlate with diabetes and inflammation indices.

doi: 10.1038/s41598-022-11942-x

Figure Lengend Snippet: Figure 1. Distribution of plasma CNTF levels according to participants’ health condition (A) and health condition and gender (B).

Article Snippet: The plasma concentrations of CNTF, CNTFRα, leptin, adiponectin, plasminogen activator inhibitor-1 (PAI-1) antigen and IL-6 were assessed using the following ELISA kits according to the manufacturer’s instructions: Human Ciliary Neurotrophic Factor ELISA kit (# CSB-E04527h, Cusabio, Houston, TX, USA); Human CNTFRα ELISA kit (# LS-F49895, LSBio, Seattle, WA, USA); Human Leptin ELISA kit (# EZHL-80SK, Millipore, MO, USA); Human Adiponectin ELISA kit (# AG-45A-0001YEK-KI01, Adipogen Life Sciences, Switzerland); Human PAI-1 ELISA Kit (# RAB0429, Sigma- Aldrich, Missouri, USA); Human IL-6 Quantikine HS ELISA kit (# HS600C, RRID:AB_2893335, R&D System, Minneapolis, MN, USA).

Techniques: Clinical Proteomics

Journal: Scientific reports

Article Title: Ciliary neurotrophic factor is increased in the plasma of patients with obesity and its levels correlate with diabetes and inflammation indices.

doi: 10.1038/s41598-022-11942-x

Figure Lengend Snippet: Figure 2. Correlation between plasma CNTF levels and clinical or haematological parameters of obesity, insulin resistance and inflammation in female subjects. Spearman correlation coefficients and 95% CI.

Article Snippet: The plasma concentrations of CNTF, CNTFRα, leptin, adiponectin, plasminogen activator inhibitor-1 (PAI-1) antigen and IL-6 were assessed using the following ELISA kits according to the manufacturer’s instructions: Human Ciliary Neurotrophic Factor ELISA kit (# CSB-E04527h, Cusabio, Houston, TX, USA); Human CNTFRα ELISA kit (# LS-F49895, LSBio, Seattle, WA, USA); Human Leptin ELISA kit (# EZHL-80SK, Millipore, MO, USA); Human Adiponectin ELISA kit (# AG-45A-0001YEK-KI01, Adipogen Life Sciences, Switzerland); Human PAI-1 ELISA Kit (# RAB0429, Sigma- Aldrich, Missouri, USA); Human IL-6 Quantikine HS ELISA kit (# HS600C, RRID:AB_2893335, R&D System, Minneapolis, MN, USA).

Techniques: Clinical Proteomics

Journal: Scientific reports

Article Title: Ciliary neurotrophic factor is increased in the plasma of patients with obesity and its levels correlate with diabetes and inflammation indices.

doi: 10.1038/s41598-022-11942-x

Figure Lengend Snippet: Figure 3. Correlation between plasma CNTF levels and clinical or haematological parameters of obesity, insulin resistance and inflammation in male subjects. Spearman correlation coefficients and 95% CI.

Article Snippet: The plasma concentrations of CNTF, CNTFRα, leptin, adiponectin, plasminogen activator inhibitor-1 (PAI-1) antigen and IL-6 were assessed using the following ELISA kits according to the manufacturer’s instructions: Human Ciliary Neurotrophic Factor ELISA kit (# CSB-E04527h, Cusabio, Houston, TX, USA); Human CNTFRα ELISA kit (# LS-F49895, LSBio, Seattle, WA, USA); Human Leptin ELISA kit (# EZHL-80SK, Millipore, MO, USA); Human Adiponectin ELISA kit (# AG-45A-0001YEK-KI01, Adipogen Life Sciences, Switzerland); Human PAI-1 ELISA Kit (# RAB0429, Sigma- Aldrich, Missouri, USA); Human IL-6 Quantikine HS ELISA kit (# HS600C, RRID:AB_2893335, R&D System, Minneapolis, MN, USA).

Techniques: Clinical Proteomics

Journal: Scientific reports

Article Title: Ciliary neurotrophic factor is increased in the plasma of patients with obesity and its levels correlate with diabetes and inflammation indices.

doi: 10.1038/s41598-022-11942-x

Figure Lengend Snippet: Figure 4. Plasma CNTFRα level distribution according to health condition (A) and health condition and gender (B). CNTF/CNTFRα molar ratio distribution according to health condition (C) and health condition and gender (D).

Article Snippet: The plasma concentrations of CNTF, CNTFRα, leptin, adiponectin, plasminogen activator inhibitor-1 (PAI-1) antigen and IL-6 were assessed using the following ELISA kits according to the manufacturer’s instructions: Human Ciliary Neurotrophic Factor ELISA kit (# CSB-E04527h, Cusabio, Houston, TX, USA); Human CNTFRα ELISA kit (# LS-F49895, LSBio, Seattle, WA, USA); Human Leptin ELISA kit (# EZHL-80SK, Millipore, MO, USA); Human Adiponectin ELISA kit (# AG-45A-0001YEK-KI01, Adipogen Life Sciences, Switzerland); Human PAI-1 ELISA Kit (# RAB0429, Sigma- Aldrich, Missouri, USA); Human IL-6 Quantikine HS ELISA kit (# HS600C, RRID:AB_2893335, R&D System, Minneapolis, MN, USA).

Techniques: Clinical Proteomics

Journal: Stem Cell Research & Therapy

Article Title: Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function

doi: 10.1186/s13287-018-0884-3

Figure Lengend Snippet: Relative messenger RNA (mRNA) expression and neurotrophin secretion levels of all groups. a The relative expression level of nerve growth factor receptor (NGFR) (p75) mRNA was not significantly different among sustaining dASCs 7d (5.55 ± 0.29), intermittent dASCs (5.11 ± 0.23), and SCs (5.54 ± 0.34), which were significantly higher ( p < 0.01) than the other three groups. b The relative expression level of P0 mRNA was highest in SCs (14.11 ± 0.88). There were no significant differences between intermittent dASCs (8.46 ± 0.34) and sustaining dASCs 7d (7.10 ± 0.54). However, intermittent dASCs showed significantly higher P0 mRNA expression ( p < 0.05) in comparison with sustaining dASCs 10d (6.00 ± 0.12) and 4d groups (2.66 ± 0.19). c The relative expression level of glial fibrillary acidic protein (GFAP) mRNA was not significantly different between intermittent dASCs (4.62 ± 0.21) and the three sustaining dASCs groups, all of which showed significant upregulation ( p < 0.01) in comparison with the undifferentiated adipose-derived stem cells (uASCs). d The levels of nerve growth factor (NGF) secreted by intermittent dASCs (99.37 ± 5.00 pg/ml) and sustaining dASCs 7d (95.20 ± 4.34 pg/ml) were significantly higher ( p < 0.01) than those of uASCs (31.18 ± 3.09 pg/ml), and sustaining dASCs 4d (54.69 ± 2.20 pg/ml) and 10d (45.90 ± 2.27 pg/ml), but lower ( p < 0.01) than that of SCs (112.46 ± 4.55 pg/ml). e , f Brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) secretion levels showed similar tendencies. The concentrations of BDNF and GDNF secreted by intermittent dASCs were 483.01 ± 10.08 and 205.66 ± 6.01 pg/ml, respectively, which were higher ( p < 0.01) than in the other groups, including SCs. g The concentrations tendency of ciliary neurotropic factor (CNTF) and NGF were similar. Data are expressed as means ± SEM. * p < 0.05, ** p < 0.01, one-way ANOVA with Tukey’s post-test or Dunnett’s T3 post-test. dASC, differentiated adipose-derived stem cell; n.s., no significant difference, SC, Schwann cell

Article Snippet: The concentrations of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), and ciliary neurotrophic factor (CNTF) in the supernatant of each group were examined by ELISA using Rat NGF/NGF beta ELISA Kit (EK0471; Boster), Rat BDNF ELISA Kit (EK0308; Boster), Rat GDNF ELISA Kit (EK0363; Boster), and Rat CNTF ELISA Kit (EK0324; Boster), respectively.

Techniques: Expressing, Comparison, Derivative Assay

Journal: Cell chemical biology

Article Title: Development of MAP4 kinase inhibitors as motor-neuron-protecting agents

doi: 10.1016/j.chembiol.2019.10.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human recombinant ciliary neurotrophic factor , Peprotech , Cat # 257-NT-050.

Techniques: Virus, Recombinant, Knock-Out, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Software